We show that aggregation method instructs EB lineage bias, with faster aggregation promoting pluripotency loss and ectoderm, and slower aggregation favoring mesoderm and endoderm.
In the process of division, plant cells cultivated in vitro in liquid nutrient media do not pass into the culture medium in the form of single cells, but they tend to maintain connections with each other, forming groups of cells of different sizes. ... Cell aggregation is affected by the presence of growth regulators in the medium. High levels ...
It considers the behavior of dissociated cells in suspension, the mechanisms and factors involved in reaggregation, and the rate and stages of this process in different representatives of this phylum.
120 rowsCell aggregates are important tools in the study of tissue development, permitting correlation of cell–cell interactions with cell differentiation, viability, and …
One of the most fundamental aspects of developmental biology is the ability of cells to aggregate and form tissue patterns. Based on a lattice model, we investigate the underlying dynamics of the aggregation process incorporating three different kinds of cellular interactions, namely, due to cell-cell adhesion mediated by physical contacts, …
Drugs that influence the aggregation process may therefore interfere with cell–cell adhesion as well as with cell growth or de-adhesion. In this way, the slow aggregation assay on agar may also become a useful tool for testing the effects of drugs on cell–substrate adhesion.
The results show that protein aggregation can be controlled during cell culture and helps to improve bioprocessing of mAbs, by giving insights into the protein aggregation at its origin in mammalian cell culture. Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these …
Pluripotent stem cells (PSC) are a promising cell source for creating therapeutics because these cells can be expanded indefinitely and differentiated into all cell types in the body. Industrializing these therapies will require the development of scalable and controlled suspension bioreactors for PSC-based cell therapy manufacturing.
The multi-step protein aggregation process. (A) Folded proteins misfold to form soluble oligomers, which proceed to accumulate and evolve into insoluble aggregates.(B) Previous methods employ FP-tagged or fluorescently labeled proteins to monitor protein aggregation.Events of aggregation are visualized by the transition from …
Cellular aggregation is a complex process orchestrated by various kinds of interactions depending on the environment. Different interactions give rise to different pathways of cellular rearrangement and the …
Based on an optimized electroporation protocol, we designed a rapid, milliliter-scale diagnostic transient production assay to identify limitations in the ability of Chinese hamster ovary (CHO) cells to produce a model "difficult-to-express" homodimeric Fc-fusion protein, Sp35Fc, that exhibited very low volumetric titer and intracellular formation of disulfide …
While heparin has been shown to eliminate cell aggregation in suspension adaptations of insect and HEK293 cells for virus-based cell cultures, the role of heparin in long period serum-free suspension adaptation of the anchorage-dependent Chinese hamster ovary (CHO) cell lines remains inconclusive. I …
1. Introduction. Cell aggregation is a dynamic and complex process ubiquitous in life. It occurs in morphogenesis, from organisms situated at the border between single and multicellular life forms, for instance Dyctyostelium discoideum [1], to mammals in which tissues are formed by the assembly of migratory and similar cells …
Although this method is suitable for aggregation of many cells, serum or serum proteins must be added to promote cell aggregation in many cases (Matsuda, 1988), making it difficult to characterize and control the aggregation process. specialized techniques can be used to produce aggregates in certain cases, principally by controlling cell ...
We have utilized the VW bioreactor in several studies [5, 20, 21] to culture hiPSCs as aggregates over multiple passages.The baseline bioprocess developed in these studies identified and systemically optimized key PIVs, such as agitation rate and environmental oxygen, and process protocols, such as inoculation and in-vessel …
Cancer cell aggregation is a key process involved in the formation of tumor cell clusters. It has recently been shown that clusters of circulating tumor cells (CTCs) have an increased metastatic potential …
Abstract Mammalian cell lines are frequently used as the preferred host cells for producing recombinant therapeutic proteins (RTPs) having post-translational modified modifications similar to those observed in proteins produced by human cells. Nowadays, most RTPs approved for marketing are produced in Chinese hamster ovary …
While heparin has been shown to eliminate cell aggregation in suspension adaptations of insect and HEK293 cells for virus-based cell cultures, the role of heparin in long period serum-free suspension adaptation of the anchorage-dependent Chinese hamster ovary (CHO) cell lines remains inconclusive. In this paper, we explore the …
Introduction. One of the most fundamental aspects of developmental biology is the ability of cells to aggregate and form tissues. Understanding the underlying mechanisms of cell-cell interactions in the aggregation process and the consequent tissue formation is immensely important to design therapeutic approaches for a number …
The role of disaggregase type chaperones is to establish a suitable balance between the aggregation process and protein solubilization and refolding. ... Curli protein is an extracellular amyloid fiber that mediates bacterial attachment to surfaces, cell-cell aggregation and biofilm formation. Aggregation is kept in check in part by five ...
S. aureus cells aggregate when pH is decreased and salt or protein concentrations are increased. (a) Aggregation of S. aureus cells as a function of pH in water with 0.015% and 0.5% of NaCl and in ...
MOAG binds to negatively charged residue clusters distal from APRs on aggregation-prone proteins and expedites aggregation into inclusions, thereby limiting toxicity of the aggregation process to ...
In this article, we present new experimental results on the follow-up of the formation of aggregates on our hydrogels, from the early stages (individual cells) to the …
Aberrantly processed or mutant proteins misfold and assemble into a variety of soluble oligomers and insoluble aggregates, a process that is associated with an increasing number of diseases that are not curable or manageable. Herein, we present a chemical toolbox, AggFluor, that allows for live cell …
Protein folding is crucial for achieving functional three-dimensional structures. However, the process is often hampered by aggregate formation, necessitating the presence of chaperones and quality control systems within the cell to maintain protein homeostasis. Despite a long history of folding stu …
Protein aggregation is a basic component of cells, the balance of protein aggregation is the key process for cells to keep protein homeostasis. In aging cells, this balance can be broken for lysosome degradation, ER stress, UPS, and so on. Also, protein aggregation accumulation could promote cell aging through breaking protein homeostasis.
A key goal in process development for antibodies is to increase productivity while maintaining or improving product quality. During process development of an antibody, titers were increased from 4 to 10 g/L while simultaneously decreasing aggregates. Process development involved optimization of medi …
2.2. Spheroid Formation In Vitro. The formation process of multicellular spheroidal aggregates in low attachment conditions starts with the initial loose cell aggregate formation through integrin-ECM binding followed by the spheroid compaction through enhanced cell to cell connection via homophilic cadherin binding [15, 23, …
The cell aggregation process gives rise to multicellular assemblies with arbitrary shapes and numbers of cells that include different types of structures based on …
The aggregation of misfolded proteins is associated with the perturbation of cellular function and ageing. However, protein aggregation can also be a regulated process that deposits aggregates at ...
Low ADP concentrations have been shown to initiate reversible platelet aggregation, with no granule release, whereas higher ADP concentrations trigger granule release and prostaglandin synthesis, leading to a typical, biphasic, irreversible aggregation process . Platelets' response to ADP is mediated by P2Y (P2Y1 and P2Y12) G protein …
1 Introduction. The formation of stable aggregates is very common in nature. For example, long-range attraction through chemotaxis can lead to aggregation of Dictyostelium cells [] or eukaryotic cells during development to form organs and blood vessels []).But the formation of aggregates can also arise from Brownian motion and …
Aggregation of cells and clusters. As often done for colloids or aerosols, we study the aggregation process with a Smoluchowski equation [47].Indeed, in many experiments on cells in suspension, such as the experiment that we consider in Section 3.2, cell aggregates grow by cell proliferation but also by successive aggregation …
Intracellular and extracellular accumulation of aggregated protein are linked to many diseases, including ageing-related neurodegeneration and systemic amyloidosis. Cells avoid accumulating potentially toxic aggregates by mechanisms including the suppression of aggregate formation by molecular chaperones and the degradation of …
Aggregation of mislocalized FUS into non-amyloid inclusions is believed to be pivotal in the development of cell dysfunction, but the mechanism of their formation is unclear. Using transi … Multistep process of FUS aggregation in the cell cytoplasm involves RNA-dependent and RNA-independent mechanisms Hum Mol Genet. 2014 …
